Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS
- 1 June 1988
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (4) , 225-228
- https://doi.org/10.1007/bf00272529
Abstract
The reporter genes for Chloramphenicolacetyltransferase (CAT), Neomycinphosphotransferase-(NPT)-II and β-Glucuronidase (GUS) were compared in transient gene expression experiments in tobacco mesophyll protoplasts. For this purpose, nearly identical chimeric genes controlled by the CaMV 35 S promoter were constructed. The detection level of each system was determined yielding the following order of relative sensitivity: CAT<NPT II<GUS.Keywords
This publication has 27 references indexed in Scilit:
- Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptakePlant Cell Reports, 1987
- Transgenic Plants as Tools to Study the Molecular Organization of Plant GenesScience, 1987
- Transient expression of electroporated DNA in monocotyledonous and dicotyledonous speciesPlant Cell Reports, 1987
- Regulation of Inducible and Tissue-Specific Gene ExpressionScience, 1987
- Transient expression of the plasmid pCaMVCAT in plant protoplasts following transformation with polyethyleneglycolExperimental Cell Research, 1987
- Chloramphenicol acetyl transferase activity in Brassica spp.Plant Science, 1987
- Transient gene expression in a Gramineae cell lineMolecular Genetics and Genomics, 1986
- Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplastsMolecular Genetics and Genomics, 1985
- A Simple and General Method for Transferring Genes into PlantsScience, 1985
- A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extractsGene, 1984