Echinomycin binding to the sequence CG(AT)nCG alters the structure of the central AT region
Open Access
- 25 April 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (8) , 1957-1963
- https://doi.org/10.1093/nar/18.8.1957
Abstract
DNA fragments containing the sequence CG(AT) n CG have been used in footprinting experiments to assess the effect of echinomycin, which binds to the CG steps, on the structure of the central AT region. DNAase I normally cuts ApT much better than TpA; in the presence of the drug this preference is retained but cleavage at TpA is enhanced. Changes in cleavage by micrococcal nuclease have also been observed. Echinomycin renders alternate adenines hyperreactive to diethylpyrocarbonate. The results suggest that echinomycin induces structural changes in regions surrounding its binding site and that these can be cooperatively propagated over several turns of the DNA helix.This publication has 22 references indexed in Scilit:
- Unstable Hoogsteen base pairs adjacent to echinomycin binding sites within a DNA duplex.Proceedings of the National Academy of Sciences, 1989
- Chemical probes reveal no evidence of Hoogsteen base pairing in complexes formed between echinomycin and DNA in solutionJournal of Molecular Recognition, 1988
- Actinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structureNucleic Acids Research, 1988
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- The Molecular Structure of a DNA-Triostin A ComplexScience, 1984
- Echinomycin Binding Sites on DNAScience, 1984
- DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprintingNucleic Acids Research, 1984
- Sequence-specific binding of echinomycin to DNA: evidence for conformational changes affecting flanking sequencesNucleic Acids Research, 1984
- A hypothesis on a specific sequence-dependent conformation of DNA and its relation to the binding of the lac-repressor proteinJournal of Molecular Biology, 1979
- The binding of echinomycin to deoxyribonucleic acidBiochemical Journal, 1976