Statistics of protein library construction
Open Access
- 2 June 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Bioinformatics
- Vol. 21 (15) , 3314-3315
- https://doi.org/10.1093/bioinformatics/bti516
Abstract
Summary: We have investigated the statistics associated with constructing and sampling large protein-encoding libraries. Using fairly simple statistics we have written algorithms for estimating the diversity in libraries generated by the most commonly used protocols, including error-prone PCR, DNA shuffling, StEP PCR, oligonucleotide-directed randomization, MAX randomization, synthetic shuffling, DHR, ADO and SISDC. Availability: Web interface and C++ source code available at http://guinevere.otago.ac.nz/stats.html Contact:aef@sanger.otago.ac.nz Supplementary information: Complete mathematical notes, model assumptions and justification, users' guide and worked examples at above website.Keywords
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