Endomysial antibody production is not related to histological damage after in vitro gluten challenge

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Abstract
Objective To determine whetherin vitroinduction of endomysial antibodies is anin vitromarker of coeliac toxicity. Design To determine whetherin vitroendomysial antibodies induced by gliadin incubation correlate with histological damage induced byin vitrogliadin challenge. Methods Small-bowel organ cultures from seven patients with treated coeliac disease were incubated in an organ culture system with gliadin; histological damage was morphometrically evaluated and endomysial antibodies were measured in the organ culture supernatant by indirect immunofluorescence. Results Although incubation with gliadin caused histological damage, there was no detectable production of endomysial antibody. Conclusions In vitro,endomysial antibody induction cannot be used as a marker of coeliac toxicity. Endomysial antibodies are not necessary for generating the histological lesion of coeliac disease.Eur J Gastroenterol Hepatol12:57 - 60 © 2000 Lippincott Williams & Wilkins European Journal of Gastroenterology & Hepatology 2000, 12:57-60

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