Amplification of HTLV-1-related Sequences among Patients with Neurological Disorders in Highly Endemic Nagasaki: Lack of Evidence for Association of HTLV-1 with Multiple Sclerosis
Open Access
- 1 March 1990
- journal article
- research article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 81 (3) , 238-246
- https://doi.org/10.1111/j.1349-7006.1990.tb02556.x
Abstract
We studied DNA sequences homologous to HTLV‐1 in peripheral blood mononuclear cells (PBMC) of 30 patients with neurological disorders in Kyushu, where HTLV‐1 is highly endemic. The regions of HTLV‐1 amplified by means of polymerase chain reaction (PCR) included U3, U5, gag, pol, env and pX. Our system specifically detected HTLV‐1 sequences only from HTLV‐1‐positive cells and was sufficiently sensitive to detect one proviral copy per 103 PBMC. All PCR‐positive cases were seropositive, including 4 cases of tropical spastic paraparesis/HTLV‐1‐associated myelopathy (TSP/HAM) (4/4), and one case each of myasthenia gravis (1/7) and multiple sclerosis (1/8). The PCR‐positive rate of patients, excluding 4 TSP/HAM cases, was 8% (2/26), which is similar to the seroprevalence of the adult population in the area. The data suggest that multiple sclerosis is not associated with prototype HTLV‐1.Keywords
This publication has 41 references indexed in Scilit:
- Performance Certification of Gelatin Particle Agglutination Assay for Anti-HTLV-1 Antibody: Inconclusive Positive ResultsJapanese Journal of Cancer Research, 1989
- Molecular Cloning of the Closed Circular Provirus of Human T Cell Leukaemia Virus Type I: A New Open Reading Frame in the gag-pol RegionJournal of General Virology, 1987
- HTLV-I ASSOCIATED MYELOPATHY, A NEW CLINICAL ENTITYThe Lancet, 1986
- Multiple sclerosis and human T-cell lymphotropic retrovirusesNature, 1985
- ANTIBODIES TO HUMAN T-LYMPHOTROPIC VIRUS TYPE-I IN PATIENTS WITH TROPICAL SPASTIC PARAPARESISPublished by Elsevier ,1985
- ANTIBODIES TO ATLV (HTLV) IN NIGERIAN BLOOD DONORS AND PATIENTS WITH CHRONIC LYMPHATIC LEUKAEMIA OR LYMPHOMAThe Lancet, 1983
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mersJournal of the American Chemical Society, 1983
- Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease.Proceedings of the National Academy of Sciences, 1982
- Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphomaProceedings of the National Academy of Sciences, 1980