Purification and Partial Characterization of the H Immobilization Antigens of Tetrahymena thermophila1
- 1 May 1986
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 33 (2) , 204-208
- https://doi.org/10.1111/j.1550-7408.1986.tb05590.x
Abstract
The H immobilization antigens specified by the SerH locus of Tetrahymena thermophila have been purified by a procedure utilizing acid fractionation, ammonium sulfate precipitation, gel filtration, and ion exchange chromatography. Purified antigen migrates as a single band on SDS-PAGE and IEF. Molecular weights of the four alleic H antigens range from 44,000 to 52,000, and isoelectric points ranges from 4.1 to 4.5. No carbohydrate was detected.This publication has 24 references indexed in Scilit:
- Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocelluloseGene Analysis Techniques, 1984
- Differential expression of immobilization antigen genes in Tetrahymena thermophila, II. Reciprocal and non-reciprocal transfer of i-antigen during conjugation and expression of i-antigen genes during macronuclear developmentCell Differentiation, 1981
- Identification and Purification of an External Antigenic Glycoprotein (Immobilization Antigen) From Pseudomicrothorax DubiusThe Journal of Protozoology, 1980
- Purification of the i-antigen 51A fromParamecium tetraurelia by immunoaffinity chromatographyImmunochemistry, 1978
- The Immobilization Antigen of Paramecium aurelia is a Single Polypeptide ChainThe Journal of Protozoology, 1975
- Immobilization antigens of Tetrahymena pyriformis*1I. Assay and extractionExperimental Cell Research, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Characterization and Distribution of “H” Serotypes in 25°C Cultures of Tetrahymena pyriformis, Variety 1.The Journal of Protozoology, 1961
- Studies on the Antigens of Paramecium aurelia with the Aid of Fluorescent AntibodiesJournal of General Microbiology, 1957
- The ciliary antigens of stock 172, Paramecium aurelia, variety 4Journal of Experimental Zoology, 1956