A ROUTINE PROCEDURE FOR THE DETERMINATION OF CATECHOLAMINES IN URINE AND TISSUES

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Abstract
A simplified procedure for the determination of catecholamines, suitable for routine laboratory use, has been developed. The whole of the adsorption–elution process, by which the catecholamines are concentrated and purified, is performed in one flask. The potentiometric titration required at this stage in the adsorption of the catecholamines on alumina is avoided by the use of phenol-phthalein as an internal indicator. The fluorimetric estimation in the second stage of the procedure is based upon lutin formation, a reaction nearly specific for adrenaline and noradrenaline. The small error resulting from the presence of hydroxytyramine in the urine is discussed. The method provides for the determination of losses encountered in the extraction process, so that each run includes an internal correction rather than an assumed average factor. Examples of the utility of the method are given. These include the determination of the rate of excretion of catecholamines in the urine, the effect of insulin on this rate, and the estimation of the catecholamine content of rat organs.

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