The Value of Immunoelectroosmophoresis (IEOP) for Etiological Diagnosis of Acute Respiratory Tract Infections due to Pneumococci and Mycoplasma Pneumoniae

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Abstract
Immunoelectroosmophoresis (IEOP) has been used to detect pneumococcal and Mycoplasma pneumoniae antigen in 324 sputum samples from 224 patients. Pneumococcal antigen was found in 30/37 samples from which pneumococci had earlier been isolated and in 72/243 specimens where they had not been found. Of these 72 samples 69 were from patients treated with antibiotics. Mycoplasma antigen was found in 9/57 sputum samples from which Mycoplasma had been isolated and in 2/32 other samples from patients with a serologically verified diagnosis of mycoplasmal infection. As to pneumococci, the IEOP is of value because of its rapidity and especially because antigen findings can be made in patients treated with antibiotics. In spite of sonication and concentration, mycoplasma antigen was too rarely found for the method, as now carried out, to be useful in diagnostic work.

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