Standards For Corrected Fluorescence Spectra

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Abstract
A set of four fluorescent standards has been produced and calibrated at the National Institute of Standards and Technology (NIST) for corrected relative spectral emission over the wavelength range from 400 to 740 nm. This new Standard Reference Material (SRM) 1931 has been produced in the form of sintered mixtures of inorganic phosphors and polytetrafluorethylene (PTFE) powder for spectral response calibration of spectrofluorimeters used in biology, medicine and studies of molecular structure. They provide a means for correcting spectrofluorimeter errors due to wavelength dependencies of optics, monochromators, and detectors, thus permitting meaningful intercomparison of results obtained in different laboratories.© (1989) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.

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