Influence of Several Growth Regulators and Amino Acids onin vitroOrganogenesis ofTorenia fournieriLind.

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Abstract
The effects of several growth regulators and amino acids on in vitro organogenesis of Torenia fournieri Lind. were determined using internodal segments. Treatment with 2,4-D1 resulted in nodular callus formation, while NAA and IAA induced roots constantly but much less frequently shoot buds. Individually BA, zeatin, and 4-PU induced bud formation, but these shoot buds did not develop further. Formation of buds by cytokinin was influenced by a simultaneous application of NAA or 2,4-D, but not of IAA, its degree being reduced when BA was simultaneously applied with NAA or 2,4-D. When zeatin or kinetin was added with NAA, numerous roots were induced. The effects of various L-amino acids on in vitro organogenesis were also investigated using the defined medium in which KNO3 was a principal source of nitrogen. The formation of buds was considerably stimulated by alanine and asparagine, and slightly by glutamic acid in the medium containing both NAA and BA, in which bud formation was easily induced. On the other hand, all amino acids except for glutamic acid and aspartic acid inhibited room formation in this medium. Root formation was greatly stimulated by proline, alanine, glutamine, glutamic acid, and aspartic acid, and slightly by arginine and tryptophan in the medium containing NAA but no BA. Glutamic acid and aspartic acid also enhanced bud formation in this medium.
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