Peptides bound endogenously by HLA‐Cw*0304 expressed in LCL 721. 221 cells include a peptide derived from HLA‐E
- 1 October 1996
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 48 (4) , 325-328
- https://doi.org/10.1111/j.1399-0039.1996.tb02652.x
Abstract
The peptide‐binding specificity of HLA–Cw*0304 was determined. Sequence analysis of endogenously‐bound peptides isolated from Cw*0304 expressed by LCL 721. 221 (221 for short) cells transfected with Cw*0304 cDNA revealed this class I allotype preferentially binds peptides possessing alanine at position 2 and leucine or methionine at the C‐terminus. One peptide isolated from Cw*0304 expressed by 221 cells has sequence identity to residues 116–126 of HLA‐E. Expression of HLA‐E by 221 cells was confirmed by isolation of mRNA transcripts for HLA‐E*0101 and detection of β2‐microglobu‐lin (β2‐m)‐associated HLA‐E protein.Keywords
This publication has 22 references indexed in Scilit:
- HLA-A2 subtypes are functionally distinct in peptide binding and presentation.The Journal of Experimental Medicine, 1995
- Overlap in the repertoires of peptides bound in vivo by a group of related class I HLA-B allotypesCurrent Biology, 1995
- HLA class I alleles of LCL 721 and 174XCEM.T2 (T2)Tissue Antigens, 1994
- Structural heterogeneity in HLA-B70, a high-frequency antigen of black populations *Tissue Antigens, 1993
- Peptide Binding to Major Histocompatibility Complex MoleculesAnnual Review of Cell Biology, 1993
- Impaired intracellular transport and cell surface expression of nonpolymorphic HLA-E: evidence for inefficient peptide binding.The Journal of Experimental Medicine, 1992
- Differential expression of HLA-E, HLA-F, and HLA-G transcripts in human tissueHuman Immunology, 1990
- Defective Presentation of Endogenous Antigen by a Cell Line Expressing Class I MoleculesScience, 1990
- Human leukocyte antigen F (HLA-F). An expressed HLA gene composed of a class I coding sequence linked to a novel transcribed repetitive element.The Journal of Experimental Medicine, 1990
- Hemizygous HLA mutants of lymphoblastoid cells: A new cell type for histocompatibility testingHuman Immunology, 1980