Association of viral 126kDa protein-containing X-bodies with nuclei in mosaic-diseased tobacco leaves

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Abstract
During the development of systemic mosaic symptoms in tobacco mosaic virus (TMV)-infected tobacco, the viral non-structural 126-kDa-protein was present among the chromatin-associated proteins in fractionated leaf homogenates [Van Telgen HJ et al. (1984) Virology 143: 612–616]. Using an antiserum raised against a fusion protein of β-galactosidase and part of the 126-kDa-protein of TMV, this viral protein was detected by immunoelectron microscopy in X-bodies in infected tissue. No labelling of nuclei was apparent. However, in embedded purified nuclear preparations from systemically infected leaves amorphous structures, most likely X-bodies, were present and specifically labelled. In contrast, using antibodies against tobacco histones, only nuclei were labelled. Antibodies against viral coat protein labelled crystalline virus inclusions in the cytoplasm and did not react with nuclei. Light microscopic analysis indicated that X-bodies were almost always associated with nuclei. Thus, the presence of X-bodies in nuclear preparations appeared to result from adherence of the X-bodies to the nuclei.