Serological Characterization of Human Monocytes for HLA, B-Lymphocyte, Granulocyte, and Monocyte-Associated Antigens by Cytotoxicity Testing

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Abstract
Highly enriched preparations of monocytes, B [bone marrow-derived] and T[thymus-derived] lymphocytes, and granulocytes from 18 normal donors were serotyped in parallel in a complement-dependent cytotoxicity assay using allogeneic and heterologous antisera defining 3 independent tissue antigen systems. HLA and B lymphocyte tissue antigens were detected on human monocytes although granulocyte antigens were absent. By cotytoxicity testing the presence of Ia[immune response-associated]-like antigens on monocytes was significantly diminished compared to the autologous B lymphocyte population and has important implications in B lymphocyte serology. The study identified a number of human antisera obtained from multitransfused subjects and pre- and post-transplant organ recipients that were non-HLA and appeared to define monocyte-associated antigens. The serological implications of surface antigen expression on human monocytes compared with other peripheral blood cells are discussed.