STUDIES ON HUMAN LEUCOCYTES IN DIFFUSION CHAMBERS ON THE CHICK CHORIOALLANTOIS

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Abstract
The response of human lymphocytes to phytohaemagglutinin and to allogeneic cells has been studied in diffusion chambers placed on the chick embryo chorioallantois. In this favourable environment, the proliferative response to phytohaemagglutinin was intense and resulted in more than a threefold increase in total lymphoid cell number over a 6-day culture period. The proliferation of “blast” cells was accompanied by an overall reduction in cell size so that after 6 days incubation many cells were no larger than small lymphocytes. A vigorous response, in terms of “blast” cell formation, has been noted in chambers containing lymphocytes of unrelated individuals and it is likely that the factors which favour an intense response to phytohaemagglutinin favour a maximal response in these suspensions also. A very low level of response was found in non-mixed lymphocyte cultures, which suggests that the component of the mixed lymphocyte response which may be directly attributable to the presence of avian antigens is small. In some mixed cultures, cells were fixed and stained in situ on the membrane filters. These preparations showed that “blast” cells were distributed in groups which increased in size with continued incubation.

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