SYNTHESIS OF GLYCOSAMINOGLYCANS BY CULTURES OF NORMAL HUMAN CORNEAL ENDOTHELIAL AND STROMAL CELLS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 17  (6) , 523-527
Abstract
Monolayer cultures of normal human corneal endothelial and stromal cells were incubated with [35S]sulfate and [3H]glucosamine for 4 h. The labeled glycosaminoglycans resulting from this incubation were isolated from the cell layer and the growth medium and further characterized. Endothelial and stromal cell cultures synthesized a variety of sulfated glycosaminoglycans, with chondroitin 6-sulfate as the major product. Chondroitin 4-sulfate, dermatan sulfate and heparan sulfate were present in smaller amounts. Keratan sulfate was produced only in minimal amounts. Both cell types also synthesized hyaluronic acid. The hyaluronic acid production in stromal cell strains derived from donors of different ages was similar. The demonstration that the endothelial cell strain derived from a 1 day old baby contained more hyaluronic acid than cultures from older donors suggested a possible age-related phenomenon, as seen in developing tissues.

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