Abstract
Incubation of (−)‐Δ1‐3, 4‐trans‐tetrahydrocannabinol (= Δ1‐THC; 3) with stationary cultures of Cunninghamella blakesleeana LENDER (Zygomycetales) (ATCC 8688a) yielded a number of metabolic conversion products. Isolation and structure elucidation of 6α‐hydroxy‐Δ1‐THC (4), the potential psychoactive 3″‐hydroxy‐Δ1‐THC (2) and 4″‐hydroxy‐Δ1‐THC (1), and the hitherto unknown metabolites 4″‐hydroxy‐6‐oxo‐Δ1‐THC (5), 4″, 6α‐dihydroxy‐Δ1‐THC (7) and 4″, 7‐dihydroxy‐Δ1‐THC (6) is described.