Characteristic fragmentations of peptide derivatives containing proline in negative‐ion fast atom bombardment mass spectrometry

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Abstract
Fragmentations of N‐benzyloxycarbonyl‐protected tripeptide ethyl esters containing proline were compared with those of the corresponding peptide derivatives not containing proline in negative‐ion fast atom bombardment mass spectrometry. The fragment ion [M – 109]− due to loss of the benzyloxy group followed by dehydrogenation from the peptide molecule was the base peak in the negative‐ion mass spectra for the peptides not containing proline, whilst it was a very weak fragment ion or not observed at all in those for the peptides containing proline. These results suggest that the fragmentations of the peptide derivatives in negative‐ion fast atom bombardment mass spectrometry depend on the conformational difference of the peptide derivatives owing to the existence of proline in the derivative.