HPLC Determination of the Sun-Screen Agent Uvinul T-150 in Cosmetic Products Using Diode-Array Detection

Abstract
A method is described for the quantitative determination of the sun-screen agent Uvinul T-150 in cosmetic products. It is based on a simple extraction into acidic THF and a HPLC determination with gradient elution and diode array detection, using a column packed with 5-μm Lichrosorb RP-18. The detection limit is 10 ng and linearity is observed up to 5μg injected. Other sun-screen agents, commonly used in the finished products, have been analyzed and their chromatographic parameters are reported. The method has been applied for determiningOvinu1 T-150 and all the compounds considered in some cosmetic samples of different polarity.