Microtubule translocation caused by three subspecies of inner‐arm dynein from Chlamydomonas flagella

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Abstract
To help understand the function of inner‐arm dynein in nagellar motility, dynein samples from an outer arm‐missing mutant of Chlamydomonas (odal) were examined for the ability to translocate microtubules in vitro. High‐salt extract ofaxonemes containing inner‐arm dynein was separated by ion‐exchange chromatography into 7 peak fractions with ATPase activities. Of these, three fractions containing different sets of dynein heavy chains translocated microtubules. The maximal velocities were all between 3 and 5 , which were comparable to the microtubule sliding rate in disintegrating oda axonemes.