Analysis of PPAR alpha function in human kidney cell line using siRNA

Open Access
Publisher Website
Google Scholar
Abstract
The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARα is mainly expressed in the liver, kidney, heart and muscle. PPARα activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARα is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARα in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARα and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARα siRNA. These results suggest that PPARα regulates fatty acid metabolism and body water homeostasis in this cell line.

This publication has 0 references indexed in Scilit: