Effect of heparin on proliferation and signalling in BC3H‐1 muscle cells

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Abstract
We studied binding and growth inhibitory properties of different glycosaminoglycans in growing and differentiated BC3H‐1 muscle cells. Heparin (10 ) and heparan sulfate (10 ) significantly inhibited DNA synthesis in growing and differentiated cells, as monitored by [3H] thymidine incorporation. Binding of heparin to BC3H‐1 cells was specific and time‐dependent. Heparan sulfate was the only glycosaminoglycan able to displace [3H]heparin (IC50, 3.2 × 10−7 M), although it was 10‐fold less effective than heparin itself (IC50 3.6 × 1O−8 M). Scatchard analysis revealed the existence of high‐affinity hepariy binding sites (Kd, 5 × 10−8 M). Furthermore, heparin inhibited serum‐induced stimulation of inositol lipid turnover. Taken together, these results indicate that heparin inhibits BC3H‐1 cell growth by interacting with the cell surface, possibly disrupting the flow of growth factor‐related mitogenic signalling.

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