A CONTRIBUTION TO PROBLEM OF ANTI-ISONIAZID FACTOR

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Abstract
It has been demonstrated that various strains of mycobacteria produce some unknown substance or complex of substances which may be detected in filtrates of culture media and which are able to destroy isoniazid as determined by a chemical method. This compound was demonstrated in culture filtrates of strains H37Rv, Ravenel, isoniazid-resistant Ravenel, 1427, photochromogen (Pkg 4), 607, and a number of freshly isolated strains of Mycobacterium tuberculosis partially susceptible and resistant to tuberculostatic drugs. The production of the anti-isoniazid factor appears to be independent of the amount of bacterial growth in a given strain. Phosphatides, waxes, fats, and mycolic acid isolated from the H37Rv strain have no influence on the isoniazid decomposition under the conditions of the present experiments. Old Tuberculin destroys isoniazid only in relatively high concentrations which hardly occur in the medium. This anti-isoniazid factor is dialyzable and can be extracted into ethyl acetate from which it may be re-extracted into the acid or hydroxide at approximately the same rate.

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