Combined single-molecule force and fluorescence measurements for biology
Open Access
- 1 January 2003
- journal article
- review article
- Published by Springer Nature in Journal of Biology
- Vol. 2 (1) , 4
- https://doi.org/10.1186/1475-4924-2-4
Abstract
Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution.Keywords
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