Cigarette Smoke Carcinogenesis: Importance of Tumor Promoters23

Publisher Website
Google Scholar
Abstract
The tumor-promoting activity of tobacco smoke condensate was examined by skin application in ICR/Ha Swiss mice. One application of 50 μg 7,12-dimethylbenz[a]anthracene (DMBA) was followed 2 weeks later by 5 times weekly application of 40 mg tobacco smoke condensate. After 573 days, 36 of 60 mice bore papillomas; 14 bore carcinomas. In a group receiving condensate only, 18 of 60 mice bore papillomas; 4 bore carcinomas. There were 8 mice with papillomas after a single dose of DMBA and repeated application of acetone. These findings reaffirm that tobacco smoke condensate is primarily a tumor-promoting agent with weak carcinogenic activity. Application of 20 μg DMBA, followed by thrice weekly application of 5 μg benzo[a]pyrene (BaP), resulted in 21 of 30 mice with papillomas and 11 with carcinomas after 462 days on test. BaP application alone at the same dose resulted in 8 of 20 mice with papillomas and 1 with a carcinoma, suggesting that the carcinogen BaP has a “promoting-like” effect after application of an initiating agent.

This publication has 0 references indexed in Scilit: