Powdered Grass as an Enrichment Medium for Acid-forming Anaerobes

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Abstract
"Greenmelk," a commercial powdered grass mixture, prepd. from the green leaves of wheat, oats, rye and barley, compares favorably with corn meal-liver as an enrichment medium for certain butyric anaerobes. Strains of Clostridium butyricum, C. pasteurianum, C. sporogenes, C. difficile, C. fallax, C. sphenoides, C. multijermentans and C. putrificum grew at least as well in the grass as in the corn-liver medium. Different strains of C. welchii and C. paraputrificum differed in their ability to grow in the grass medium. C. acetobutylicum, C. tertium, C. botulinum, C. bifermentans and C. aerojoetidum grew in the corn meal-liver but not in the grass media. The medium was prepd. by adding 30 g. of powdered grass, 20 g. CaCO3 and 30-50 g. of either corn meal or corn starch, or 0.03% agar. No additional methods for bringing about anaerobic conditions are required.

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