Decrease in Free Cystine Content of Cultured Cystinotic Fibroblasts by Ascorbic Acid
- 13 December 1974
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 186 (4168) , 1040-1042
- https://doi.org/10.1126/science.186.4168.1040
Abstract
The 100-fold increase in free cystine content characteristic of cultured skin fibroblasts from patients with nephropathic cystinosis was decreased more than 50 percent by addition of L-ascorbic acid to the culture medium at concentrations of 0.29 to 2.9 millimolar. Fresh ascorbic acid must be added to the culture medium daily to produce a progressive decrease of the free cystine content of the cells over a 3-day period. Upon removal of ascorbic acid from the medium, the free cystine content returns to its initial value.Keywords
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