Comparative Evaluation of Semiautomated COBAS AMPLICOR Hepatitis B Virus (HBV) MONITOR Test and Manual Microwell Plate-Based AMPLICOR HBV MONITOR Test
Open Access
- 1 February 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (2) , 758-761
- https://doi.org/10.1128/jcm.39.2.758-761.2001
Abstract
Comparative evaluation of the semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR Test (COBAS-HBV) and manual AMPLICOR HBV MONITOR Test (AMPLICOR-HBV) on 208 serum samples revealed no significant difference in the sensitivities of the two assays. Twenty samples tested HBV DNA negative and 183 samples tested HBV DNA positive by both assays. Three samples tested positive by COBAS-HBV only and two samples tested positive by AMPLICOR-HBV only. HBV DNA concentrations determined by the two assays were significantly related ( n = 183, r = 0.97, P < 0.0001), which indicates that COBAS-HBV could replace AMPLICOR-HBV. The major inconvenience of COBAS-HBV is the required performance of appropriate predilutions of high-titer samples in order to extend the narrow dynamic range of the assay.Keywords
This publication has 29 references indexed in Scilit:
- A multicenter study evaluation of the digene hybrid capture II signal amplification technique for detection of hepatitis B virus DNA in serum samples and testing of EUROHEP standards.2000
- Identification of Different States of Hepatitis B Virus Infection with a Quantitative PCR AssayClinical and Diagnostic Laboratory Immunology, 2000
- Routine detection and quantification of hepatitis B virus DNA in clinical laboratories: performance of three commercial assaysJournal of Virological Methods, 2000
- Quantification of intrahepatic hepatitis B virus (HBV) DNA in patients with chronic HBV infectionHepatology, 2000
- The role of HBV DNA quantitative PCR in monitoring the response to interferon treatment in chronic hepatitis B virus infectionJournal of Hepatology, 1999
- What technique should be used for routine detection and quantification of HBV DNA in clinical samples?Journal of Virological Methods, 1997
- Quantitation of HBV DNA in Human Serum Using a Branched DNA (bDNA) Signal Amplification AssayAmerican Journal of Clinical Pathology, 1995
- Outcome Of Perinatal Hepatitis B Virus Exposure Is Dependent On Maternal Virus LoadThe Journal of Infectious Diseases, 1994
- Quantitative detection of hepatitis B virus DNA in serum using chemiluminescence: comparison with radioactive solution hybridization assayJournal of Virological Methods, 1994
- Detection of serum hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay.Proceedings of the National Academy of Sciences, 1989