The effect of triethylenetetramine dihydrochloride on the in vivo susceptibility of Pseudomonas aeruginosa to gentamicin.
- 1 January 1979
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 32 (8) , 834-838
- https://doi.org/10.7164/antibiotics.32.834
Abstract
A chelating agent, triethylenetetramine dihydrochloride (TRIEN dihydrochloride) increased the efficacy of gentamicin in vivo against a clinical isolate of P. aeruginosa, designated Ps 15. Mice which were inoculated with 10 .times. LD50 of Ps 15 and treated with doses of 2-16 mg of gentamicin/kg per day all died. Treatment with 8 mg of gentamicin/kg body weight per day plus 30 mg of TRIEN dihydrochloride/day markedly reduced the mortality. The combined therapy also reduced the number of viable organisms that accumulated in the kidney during a 24 h period post inoculation. When a dosage level of 8 mg of gentamicin was exceeded in the combined treatment regimen, all of the infected mice died, and a high concentration of endotoxin could be detected in the mouse sera by the Limulus assay.This publication has 0 references indexed in Scilit: