High-mannose oligosaccharides from human Tamm-Horsfall glycoprotein

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Abstract
The occurrence of high-mannose oligosaccharides on Tamm-Horsfall glycoprotein prepared from human pooled urine is reported. The Pronase digest of the glycoprotein was fractionated by gel filtration and a high-mannose glycopeptide species was separated from complex-type glycopeptides. When high-mannose glycopeptides were digested with endo-.beta.-N-acetylglucosaminidase H, followed by reduction with [3H]KBH4, 3 oligosaccharides were resolved by TLC. On the basis of chromatographic mobility and exoglycosidase digestions, the composition Man7-, Man.epsilon.-, and Man5-GlcNAc was assigned to the 3 oligosaccharides. Man6GlcNAc is by far the major component.