Nucleotide sequence of the origin of replication in bacteriophage ΦX174 RF DNA

Abstract
The gene A protein of bacteriophage .PHI.X174 was used in vitro to convert .PHI.X RF[replicative form]I DNA into the relaxed RFII form by nicking the viral strand. The nucleotide sequence at the 3'' end of the nick was determined as.sbd.T G C T C C C C C A A C T T GOH. This sequence gives the exact position of the origin of .PHI.X RF DNA replication.