Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of its Application

Abstract
A comparison of 2 derivatives of fluorescein conjugated by 3 methods was evaluated in the study of bacterial, viral and mycotic agents. Fluorescein isothiocyanate (Riggs) was much superior to fluorescein isocyanate in ease of preparation, stability and intensity of staining. Less denaturation of the globulin antibody occurred in the process of conjugation when the isothiocyanate preparation was used. A simplified technique for conjugation of the isothiocyanate compound is described.