Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of its Application
- 1 August 1958
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 98 (4) , 898-900
- https://doi.org/10.3181/00379727-98-24222
Abstract
A comparison of 2 derivatives of fluorescein conjugated by 3 methods was evaluated in the study of bacterial, viral and mycotic agents. Fluorescein isothiocyanate (Riggs) was much superior to fluorescein isocyanate in ease of preparation, stability and intensity of staining. Less denaturation of the globulin antibody occurred in the process of conjugation when the isothiocyanate preparation was used. A simplified technique for conjugation of the isothiocyanate compound is described.Keywords
This publication has 5 references indexed in Scilit:
- The formation of fowl plague virus antigens in infected cells, as studied with fluorescent antibodiesVirology, 1957
- STAINING TOXOPLASMA GONDII WITH FLUORESCEIN-LABELLED ANTIBODYThe Journal of Experimental Medicine, 1957
- Studies on canine distemper infection by means of fluorescein-labeled antibodyVirology, 1957
- STAINING BACTERIAL SMEARS WITH FLUORESCENT ANTIBODYJournal of Bacteriology, 1956
- LOCALIZATION OF ANTIGEN IN TISSUE CELLSThe Journal of Experimental Medicine, 1950