Characteristics of Extracts from Interferon-treated HeLa Cells: Presence of a Protein Kinase and Endoribonuclease Activated by Double-stranded RNA and of an Inhibitor of mRNA Methylation
- 1 December 1977
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 37 (3) , 535-546
- https://doi.org/10.1099/0022-1317-37-3-535
Abstract
Extracts from mouse interferon-treated mouse cells (Ehrlich ascites tumor and [fibroblast] L929) were reported earlier to differ in several characteristics from extracts of untreated cells. The effect of treatment with human interferon of cells of a human [cervical carcinoma] line (HeLa S3) is similarly manifested in the cell extract. A comparison of extracts from interferon-treated HeLa S3 cells (S30INT) with that from untreated cells (S30C) revealed: an impairment in S30INT of the methylation of (unmethylated) reovirus mRNA by the cellular and virus enzymes; a faster degradation of reovirus mRNA in S30INT but only in reaction mixtures supplemented with double-stranded RNA and ATP; an increased phosphorylation by ATP of 1 (or 2) proteins in S30INT but only in reaction mixtures supplemented with double-stranded RNA; and a more pronounced inhibition of translation by double-stranded RNA in S30INT. No such effects were observed in extracts prepared from HeLa cells treated with mouse interferon.This publication has 11 references indexed in Scilit:
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