Protoplast water content of bacterial spores determined by buoyant density sedimentation
- 1 August 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 163 (2) , 735-737
- https://doi.org/10.1128/jb.163.2.735-737.1985
Abstract
Protoplast wet densities (1.315-1.400 g/ml), determined by buoyant density sedimentation in Metrizamide gradients, were correlated inversely with the protoplast water contents (26.4-55.0 g of water/100 g of wet protoplast) of 9 diverse types of pure lysozyme-sensitive dormant bacterial spores. The correlation equation provided a precise method for obtaining the protoplast water contents of the other spore types with small impure samples and indicated that the average protoplast dry density was 1.460 g/ml.This publication has 10 references indexed in Scilit:
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