Antibodies to interleukin 2. Effects on immune responses in vitro and in vivo.

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Abstract
Antibodies to highly purified mouse interleukin 2 (IL-2) were raised in rabbits; a 1:500 dilution of antiserum completely blocked the in vitro mitogenic effect of 10-9 M IL-2. The antisera functioned effectively to immunoprecipitate biosynthetically labeled IL-2 and the purified Ig were useful in the construction of affinity columns for the adsorption and 1-step immunopurification of IL-2. The antibodies were apparently specific for IL-2 among the lymphokines; they did not block the biological effects of IL-1, IL-3, .gamma.-interferon, B cell stimulating factor(s) and cytotoxic T cell differentiation factor(s). When anti-IL-2 was added to the in vitro reactions, it blocked mixed leukocyte reactions (MLR) and associated lymphocyte proliferation, the in vitro generation of cytotoxic T cells, and antibody formation as assessed by erythrocyte-specific plaque-forming cells (PFC). When injected into mice, anti-IL-2 antibodies also reduced the formation of cytotoxic lymphocytes in response to allogeneic cells, suggesting that endogenous IL-2 participates in such reactions in vivo. These IL-2 antibodies should be useful adjuncts in the analysis of immune response in vivo and in vitro.

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