Osmotic volume change of cytoplasmic drops isolated in vitro from internodal cells of Characeae

Abstract
Osmotic volume change was measured for drops of protoplasm isolated from internodal cells of Chara australis in inorganic salt solutions of various osmolalities. A drop was found to maintain a definite volume in medium of a definite concentration in such a way that VAN'T HOFF'S law practically holds. This indicates (1) that the surface membrane surrounding the isolated drop is semipermeable, and (2) that practically all the space of the protoplasmic drop is osmotically active.

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