Studies on the Metabolism of Steroid Hormones and Their Precursors by the Human Placenta at Various Stages of Gestation. II.In VitroMetabolism of 3β-Hydroxy androst-5-en-17-one

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Abstract
The in vitro metabolism of 3β-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) by human placentae of various gestational ages was studied by incubation of 7α-3H-dehydroepiandrosterone with a total of 39 samples of placentae obtained at from 4 to 24 weeks of gestation, and extraction and identification of the resulting radiolabeled metabolites by reverse isotope dilution techniques. Metabolites obtained under the in vitro conditions employed include estra-1,3,5(10)-trien-3,17β-diol, 3-hydroxyestra-1,3,5(10)-trien-17-one, 2,3-dihydroxyestra-1,3,5(10)-trien-17-one, androst-4-ene-3,17-dione, 17β-hydroxyandrost-4-en-3-one, 5α-androstane-3,17-dione, 5α-androstane-3β,17β-diol, androst-5-ene-3β,17β-diol, 30-hydroxy-5α-androstan-17-one, and unaltered dehydroepiandrosterone. When the relative capacities of the various placental samples to carry out the observed metabolic transformations of the steroid substrate under the in vitro conditions employed are compared, it is seen that the differences in the results obtained with placentae of various ages are primarily of a quantitative, rather than a qualitative, nature.

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