Labelling of phospholipids from inorganic [32P]phosphate in brain preparations. Effect of acetylcholine, chlorpromazine and azacyclonol

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Abstract
The addition of chlorpromazine (0.1 mM) or azacyclonol (1 mM) increased the incorporation of inorganic [32p] phosphate into the phospholipids of slices of guinea-pig brain without any increase in the consumption of O, production of lactic acid, concentration and specific radioactivity of adenosine triphosphate (ATP) or concentration of phosphocreatine. The increase in labelling was confined to phosphatidic acid, phosphatidyl-inositol and phosphatidylserine. Higher concentrations of chlorpromazine (1 mM) and azacyclonol (5 mM) caused a decrease in the labelling of phospholipids, accompanied by a reduction in the consumption of O, concentration and specific radioactivity of ATP and concentration of phosphocreatine. The results are discussed in relation to the mechanism whereby the addition of these substances causes an increase in the labelling of phospholipids.