Demonstration of two unique metabolites of arachidonic acid from phorbol ester-stimulated bovine lymphocytes

Abstract
Treatment of bovine lymphocytes with phorbol esters activates an oxygen-dependent system for the formation of a class of arachidonic acid metabolites which appears distinct from prostaglandins, prostacyclins, thromboxanes and leukotrienes. A full activation of the system is achieved within minutes by 10−7 M 12-O-tetradecanoylphorbol-13-acetate. This activation can be largely prevented by the lipoxygenase inhibitors, 5,8,11,14-eicosatetraynoic acid and nordihydroguaiaretic acid.