Rapid freezing of deep cerebral structures for electron microscopy

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Abstract
A method is described for the investigation of deep cerebral structures by freeze‐substitution. The head of a mouse is sectioned in a guillotinelike apparatus. The exposed surface is subjected to freeze‐substitution. Electron micrographs prepared from this material exhibit under a layer of sheared tissue, a layer of tissue comparable with that observed previously in micrographs of the cerebral and cerebellar cortices subjected to freeze‐substitution of their natural surfaces. By varying the plane in which the head is sectioned any structure in the brain can be made accessible to freeze‐substitution.