The size of the splenium of the rat corpus callosum: Influence of hormones, sex ratio, and neonatal cryoanesthesia

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Abstract
The splenium (posterior ⅕) of the corpus callosum is sexually dimorphic in the adult rat brain. In the present study we examined the role of developmental hormones and cryoanesthesia (which is normally used during the performance of neonatal hormone manipulations) on the gross size of the splenium in male and female rats. There was a sex difference in splenial size (male > female) among nonhormonally manipulated animals, p = .0007. While neonatal castration was ineffective in altering the size of the male splenium, testosterone injections in females were found to increase the size of the splenium relative to oil-injected females, p = .05. The effect of developmental testosterone was further observed: Sex ratio (males to females) of the litter correlated with splenial area in females, r = .55, p = .03. Duration of cryoanesthesia negatively correlated with splenial area in males, r = −.81, p = .03, with a similar trend in females. © 1998 John Wiley & Sons, Inc. Dev Psychobiol 33: 295–303, 1998