A ROLE FOR METHYLATED BASES IN THE AMINO ACID ACCEPTOR FUNCTION OF SOLUBLE RIBONUCLEIC ACID
- 1 November 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 52 (5) , 1233-1238
- https://doi.org/10.1073/pnas.52.5.1233
Abstract
The soluble ribonucleic acid of Escherichia coli. deficient in methyl groups, shows a markedly decreased capacity to be saturated with leucine by the activating enzyme from yeast. However, the E. coli aminoacyl-sRNA synthetase can attach as much leucine to methyl-deficient sRNA as to normally methylated sRNA. It is suggested from these data that the yeast amino acid activating enzyme requires that sRNA be methylated for maximal acylation, while the E. coli enzyme has no such requirement. Analysis of normal and methyl-deficient leucyl-sRNA on methlyated albumin-kieselguhr columns has demonstrated a fraction of leucine acceptor activity that is present only in samples of methyl-deficient sRNA. This suggests that sRNA species deficient in methylated bases may be separated from the methylated species by this technique.Keywords
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