A model for the molecular organization of cytochrome β‐561 in chromaffin granule membranes

Abstract
The CD spectrum of reduced cytochrome (cyt.) β-561 in chromaffin granule membranes resembles that of mitochondrial cyt. β1 and indicates possible heme-heme interaction in the protein. Based on spectroscopic data and analysis of the amino acid sequence, a model of cyt. β-561 is suggested, in which the protein carries two transmembrane-localized hemes, each coordinated by two histidines. The model accounts for the presence of two different forms of cyt. β-561 in chromaffin granule membranes and provides a mechanism of transmembrane electron transfer by this hemoprotein.

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