CYP98A6 from Lithospermum erythrorhizon encodes 4‐coumaroyl‐4′‐hydroxyphenyllactic acid 3‐hydroxylase involved in rosmarinic acid biosynthesis1
- 13 March 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 514 (2-3) , 219-224
- https://doi.org/10.1016/s0014-5793(02)02368-2
Abstract
Rosmarinic acid is the dominant hydroxycinnamic acid ester accumulated in Boraginaceae and Lamiaceae plants. A cytochrome P450 cDNA was isolated by differential display from cultured cells of Lithospermum erythrorhizon, and the gene product was designated CYP98A6 based on the deduced amino acid sequence. After expression in yeast, the P450 was shown to catalyze the 3-hydroxylation of 4-coumaroyl-4′-hydroxyphenyllactic acid, one of the final two steps leading to rosmarinic acid. The expression level of CYP98A6 is dramatically increased by addition of yeast extract or methyl jasmonate to L. erythrorhizon cells, and its expression pattern reflected the elicitor-induced change in rosmarinic acid production, indicating that CYP98A6 plays an important role in regulation of rosmarinic acid biosynthesis.Keywords
This publication has 24 references indexed in Scilit:
- Molecular cloning and functional identification of (+)‐δ‐cadinene‐8‐hydroxylase, a cytochrome P450 mono‐oxygenase (CYP706B1) of cotton sesquiterpene biosynthesisThe Plant Journal, 2001
- CYP98A3 from Arabidopsis thaliana Is a 3′-Hydroxylase of Phenolic Esters, a Missing Link in the Phenylpropanoid PathwayJournal of Biological Chemistry, 2001
- Synthesis of 2-O-(4-Coumaroyl)-3-(4-hydroxyphenyl)lactic Acid, an Important Intermediate of Rosmarinic Acid Biosynthesis.CHEMICAL & PHARMACEUTICAL BULLETIN, 2001
- Effect of Oral Treatment of Perilla frutescens and Its Constituents on Type-I Allergy in Mice.Biological & Pharmaceutical Bulletin, 2001
- Lycopene Synergistically Inhibits LDL Oxidation in Combination with Vitamin E, Glabridin, Rosmarinic Acid, Carnosic Acid, or GarlicAntioxidants and Redox Signaling, 2000
- MOLECULAR-GENETIC ANALYSIS OF PLANT CYTOCHROME P450-DEPENDENT MONOOXYGENASESAnnual Review of Plant Biology, 1998
- Cytochrome P450-dependent hydroxylation in the biosynthesis of rosmarinic acid in ColeusPhytochemistry, 1997
- Characterization of rosmarinic acid synthase from cell cultures of Coleus blumeiPhytochemistry, 1991
- Induction of rosmarinic acid accumulation in cell suspension cultures of Orthosiphon aristatus after treatment with yeast extractPhytochemistry, 1991
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976