Single-Step Purification of “Kall Ikrein-Resistant” Kininogen from Rat Plasma Using Monoclonal-Antibody Immunoaffinity Chromatography
- 1 August 1985
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 15 (3) , 159-169
- https://doi.org/10.1080/10826068508062269
Abstract
Monoclonal antibody to rat plasma kininoqen, obtained after immunization of mice with the kininogen prepared by conventional methods, was purified from ascites fluid and coupled to CNBr-activated Sepharose-4B. Monoclonal-antibody affinity adsorbant thus prepared provided a rapid singe-step method of purifying to homogeneity plasma kininogen. Purified rat plasma kininogen showed identical molecular weight and immunological cross-reactivity to rat plasma low molecular weight (LMW) kininogen purified by conventional procedures. Rat plasma kininogen differed from LMW kininogen from other species by virtue of its resistance to cleavage by either plasma or glandular kallikreins.This publication has 25 references indexed in Scilit:
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