Production of Monoclonal Antibodies to Peanut Mottle Virus and Their Use in Enzyme-Linked Immunosorbent Assay and Dot-Immunobinding Assay

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Abstract
Stable hybridoma cell lines secreting monoclonal antibodies (MAB) to peanut mottle virus (PMV) were produced by fusing spleen cells of immunized mice and mouse myeloma cell line P3X63Ag8.653. Hybridoma clones produced antibodies of the IgG1 subclass. The MAB reacted to nine isolates of PMV but did not react to eight other viruses tested. The immunoreactivity of the MAB was compared with polyclonal rabbit serum for detection of PMV in foliar tissue by enzyme-linked immunosorbent assay (ELISA) and a dot-immunobinding assay. An ELISA using both polyclonal antibodies and MAB was superior to assays using either source of antibody alone. However, a dot-immunobinding asay using only MAB was satisfactory for detection of PMV.