The Effect of Front Size on Electron Capture Detector Sensitivity

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Abstract
Steroid samples extracted from silica gell thin-layer plates contain impurities which have relatively short retention times (sample front) on gas chromatography. Such impurities decrease the sensitivity of the electron capture cell for steroid quantification. This decrease in sensitivity at every point in time along the recording was inversely proportional to an increase in baseline height caused by the material extracted from silica gel. It was, moreover, shown that in the presence of material contributing to the width of the sample front, the technique of internal standardization may not adequately correct for the change in sensitivity induced by a wide front unless the internal standard and the steroid being quantitated came off the column close together and in a position where the baseline height did not change rapidly.

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