Evidence that the BBA68 Protein (BbCRASP-1) of the Lyme Disease Spirochetes Does Not Contribute to Factor H-Mediated Immune Evasion in Humans and Other Animals
- 1 May 2006
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 74 (5) , 3030-4
- https://doi.org/10.1128/iai.74.5.3030-3034.2006
Abstract
BBA68 (BbCRASP-1) of the Lyme disease spirochetes binds human factor H (FH) and FH-like protein 1 (FHL-1). Here we assess transcription of the BBA68 gene and production of BBA68 in infected mice and humans using real-time reverse transcriptase PCR and immunoblotting. The species specificity of FH binding to BBA68 was also tested. The data suggest that BBA68 does not play an important role in immune evasion in animals.Keywords
This publication has 34 references indexed in Scilit:
- Demonstration of OspC Type Diversity in Invasive Human Lyme Disease Isolates and Identification of Previously Uncharacterized Epitopes That Define the Specificity of the OspC Murine Antibody ResponseInfection and Immunity, 2005
- Demonstration of Factor H-Like Protein 1 Binding toTreponema denticola, a Pathogen Associated with Periodontal Disease in HumansInfection and Immunity, 2005
- Human Lyme Arthritis and the Immunoglobulin G Antibody Response to the 37-Kilodalton Arthritis-Related Protein of Borrelia burgdorferiInfection and Immunity, 2005
- Putative Coiled-Coil Structural Elements of the BBA68 Protein of Lyme Disease Spirochetes Are Required for Formation of Its Factor H Binding SiteJournal of Bacteriology, 2005
- Combined Effects of Blood and Temperature Shift on Borrelia burgdorferi Gene Expression as Determined by Whole Genome DNA ArrayInfection and Immunity, 2004
- Quantitative analysis of Borrelia burgdorferi gene expression in naturally (tick) infected mouse strainsMedical Microbiology and Immunology, 2004
- Artificial‐infection protocols allow immunodetection of novel Borrelia burgdorferi antigens suitable as vaccine candidates against Lyme diseaseEuropean Journal of Immunology, 2003
- Factor H family proteins: on complement, microbes and human diseasesBiochemical Society Transactions, 2002
- Regulation of OspE-Related, OspF-Related, and Elp Lipoproteins of Borrelia burgdorferi Strain 297 by Mammalian Host-Specific SignalsInfection and Immunity, 2001
- Mutation and Recombination in the Upstream Homology Box-Flanked ospE -Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during InfectionInfection and Immunity, 2000