Inhibition of Cytopathogenic Effect of Poliomyelitis Viruses in Tissue Culture by Antibiotic M-8450

Publisher Website
Google Scholar
Abstract
Addition of a mold filtrate (antibiotic M-8450) to tissue cultures (on monkey testicular cells in plasma clot roller tubes) of the 3 immunological types of poliomyelitis viruses protected the cells against the destructive action of all 3 types of virus when it was administered 24 hrs. before virus, but not just 10 min. before, or simultaneously with the virus. No such inhibition could be demonstrated by contact tests in which the virus and M-8450 were mixed and incubated together for 24 hrs. before being placed in culture. Viable virus could be recovered from the undamaged M-8450 treated cultures thus further indicating the effect of the antibiotic to be on the cells rather than on the virus. No toxic or inhibitory effect of the antibiotic on the cells was detected.