Use oft-butyldimethylchlorosilane/imidazole reagent for identification of molecular species of phospholipids by gas-liquid chromatography mass spectrometry

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Abstract
The t-butyldimethylsilyl derivatives of 1,2-dialkyl, 1-alk-1′-enyl-2-acyl, 1-alkyl-2-acyl and 1,2-diacyl glycerols were analysed with a gas chromatograph mass spectrometer system. The characteristic fragment ions were as follows. The molecular weight determining ion was [M - 57]+, which was formed by cleavage of the t-butyl radical from the molecular ion. The nature of the alk-1′-enyl residue could be determined by the presence of ions at [RCHCH + 56]+ and [RCHCH + 130]+ (RCHCHalk-1′-enyl), and the alkyl residue by the ion at [R + 130]+(R = alkyl group). Ions giving information about the acyl group, [RCO]+, [RCO + 74]+ and [M  RCHCHO, RO or RCOO]+ were also observed. The mass spectra of pairs of trimethylsilyl and t-butyldimethylsilyl derivatives showed differences in several respects. The t-butyldimethylsilyl derivatives gave more effective information for elucidating the structure of phosphoglycerides.