Recharacterization of fungal dinucleoside polyphosphate (HS3)

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Abstract
Three polyphosphorylated dinucleosides [Achlya sp.] given the pseudonyms of HS3, HS2 and HS1 that were erroneously described as diguanosine polyphosphates were repurified and partially recharacterized. They were extremely complex molecules; chemical (HCl and KOH hydrolysis), physical (UV-light spectral analysis and ion-exchange chromatography), and enzymic (nucleotide pyrophosphatase and bacterial alkaline phosphatase hydrolysis) studies showed that all 3 HS compounds are uracil rich and only HS3 contains a purine nucleoside and glutamate. The partial structure of HS3 was deciphered as a moiety of ADP-sugar X-glutamate (the mode of attachment of glutamate is obscure) that is covalently linked to another moiety composed of UDP, mannitol and 4 phosphates. Sugar X had chromatographic characteristics of ribitol, but the chromatographic isolate also contained a ninhydrin-sensitive entity presumed to be an amino group. Sugar X, therefore, may be an amino sugar polyol. Only the general chemical compositions of HS2 and HS1 were determined. Each contained 2 uridines and HS2 had 10 phosphates whereas HS1 had 12.